| Assay Method Information | |
| | MT Kinetic Assay |
| Description: | The assay was performed as previously described with slight modifications [Chen et al., J. Agric. Food Chem., 50:4108-12]. The reaction medium was 1 mL in 50 mM phosphate buffer (pH 6.8). The final concentration of mushroom tyrosinase was 26.07 μg/mL for cresolase activity and 6.52 μg/mL for catecholase activity. Freshly prepared enzyme, substrate, and dithiocarbamates I and II were used in this work. The reaction was carried out under constant temperatures of 27 and 37°C. Substrate addition followed incubation of the enzyme with different concentrations of synthetic inhibitors. |
| Affinity data for this assay | |
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