| Assay Method Information | |
| | HDAC Activity Assay |
| Description: | In vitro bioactivity evaluation of compounds 4a-n was performed by HDAC activity assays using a HDAC colorimetric activity assay kit (AK501; Biomol Research Laboratories). The source of HDACs was HeLa nuclear extracts, including HDAC1 and HDAC2. Assays were performed according to kit instructions. |
| Affinity data for this assay | |
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