| Assay Method Information | |
| | Acetylcholinesterase Assay |
| Description: | Total volume of the reaction mixture was 100 µL. It contained 60 µL Na2HPO4 buffer with concentration of 50 mM and pH 7.7. 10 µL test compound (0.5 mM well-1) was added, followed by the addition of 10 µL (0.005 unit well-1) enzyme. The contents were mixed and pre-read at 405 nm. Then contents were pre-incubated for 10 min at 37 °C. The reaction was initiated by the addition of 10 µL of 0.5 mM well-1 substrate (acetylthiocholine iodide), followed by the addition of 10 µL DTNB (0.5 mM well-1). After 15 min of incubation at 37 °C absorbance was measured at 405 nm using 96-well plate reader Synergy HT, Biotek, USA. All experiments were carried out with their respective controls in triplicate. Eserine (0.5 mM well-1) was used as a positive control. |
| Affinity data for this assay | |
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