| Assay Method Information | |
| | DPPH Radical Scavenging Assay |
| Description: | Preparation of DPPH solution was adopted from Molyneux [J. Sci. Technol. 26(2):211-219] and Blois [Nature 181(4617):1199-1200] with minor modification. All the test compounds were dissolved in 95% ethanol. Briefly, 0.5 mL of test compounds were added (0 - blank control, 10, 25, 50, 100, 250, 500 and 1000 µg/mL) to 0.5 mL of DPPH (2 µM in 95% ethanol) and the mixture was incubated at room temperature for 30min. The absorbance was measured at 517nm [J. Pharm. Res. 2(9):1370-1372], and the percentage inhibition of test compounds was calculated using the following equation using Microsoft Excel software (version 2010). Ascorbic acid was used as the positive control [Int. J. Chem. Sci. 7(2):1117-1126]. |
| Affinity data for this assay | |
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