| Assay Method Information | |
| | AChE Inhibitory Assay |
| Description: | In brief, reaction mixture composed of 10 µL of test sample of five different concentrations, 145 µL phosphate buffer 200 mM (pH 7.7), 80 µL of dithiobisnitrobenzoic acid (DTNB) (18.5 mg of DTNB dissolved in 10 mL phosphate buffer pH 7.7), and 10 µL of enzyme (0.4 U/mL). The mixture was incubated at 25 °C for 5 min. Subsequently, the enzymatic reaction was initiated by addition of 15 µL of 1 mM of acetylthiocholine iodide or butyrylthiocholine iodide (according to the respective enzyme), and the mixture was again incubated for 5 min at 25 °C. The rate of absorbance change was measured at 412 nm for 6 min using a microplate reader (Bio-Rad 680, Germany). Galantamine was applied as positive drug. |
| Affinity data for this assay | |
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