| Assay Method Information | |
| | DPP-IV Inhibition Screening Assay |
| Description: | DPP-IV inhibition was measured by DPP-IV inhibitor screening assay kit (Cayman Chemical Company, Item No. 700210) following the manufacturer's instructions. Briefly, solutions of test compounds at varying concentrations were prepared in dimethyl sulfoxide (DMSO) and then diluted into assay buffer. Human recombinant DPP-IV was added to the dilutions and preincubated for 30 min at 37 °C before the reaction was initiated by the addition of fluorogenic substrate solution AMC (Gly-Pro-Aminomethylcoumarin). Human recombinant DPP-8 or DPP-9 was added to the dilutions and preincubated for 10 min at 22 °C before the reaction was initiated by the addition of fluorogenic DPP substrate. The kinetics of the reaction wasmonitored (excitation at 380 nm, emission at 460 nm). |
| Affinity data for this assay | |
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