| Assay Method Information | |
| | Neuraminidase Inhibition Assay |
| Description: | A fluorogenic assay was used to measure influenza virus neuraminidase activity. The substrate, 4-methylumbelliferyl-N-acetylneuraminic acid, is cleaved by neuraminidase to yield a fluorescent product that can be quantified. Fluorescence measurements were obtained using a fluorescence plate reader with excitation and emission filters of 355 +/- 35 nm and 460 +/- 25 nm. The Ki values were calculated by nonlinear regression curve fitting of the velocity data using Kaleidagraph software. |
| Affinity data for this assay | |
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