| Assay Method Information | |
| | Competitive Binding Assay |
| Description: | Fluorescence polarization competitive binding assays were used to measure the IC50s of compounds binding to the different SH2 domains. The difference in polarization values of fluorescein-conjugated peptide reflects the bound and unbound state of the probe. IC50s were calculated based on the binding of the fluorescein-conjugated peptide to the protein with compound addition relative to vehicle-alone control samples. |
| Affinity data for this assay | |
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