| Assay Method Information | |
| | MMP2 Inhibition Assay |
| Description: | The synthesized compounds were assayed for the inhibitory activities against MMP-2 in 96-well microtiter plates using succinylated gelatin as the substrate. The compound and enzyme were dissolved in sodium borate buffer (pH 8.5, 50 mM) and incubated at 37 °C for 30 min. The substrate was added and incubated at 37 °C for another 60 min. The 100% and blank groups were also carried out, in which the 100% group contained not compound and the blank group contained only the enzyme. Then, 0.03% picrylsulfonic acid solution was added and incubated at room temperature for additional 20 min. The resulting solutions were measured under 450 nm. |
| Affinity data for this assay | |
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