| Assay Method Information | |
| | Monoamine Oxidase Inhibition Assay |
| Description: | Briefly, a crude rat brain mitochondrial suspension (male Sprague Dawley rats weighing 180 220 g, killed by decapitation) was used as protein source. Serotonin(5-HT, 100 µM) and 4-dimethylaminophenethylamine (DMAPEA, 5 µM) were used as selective substrates for MAO-A and MAO-B, respectively. The reaction mixtures were analyzed with a Merck-Hitachi HPLC (Tokyo, Japan), employing a C18 reverse phase column (Lichrospher 250 9 4.6 mm, 5 µm) and an amperometric detector (Merck Recipe L3500A). Tetrahydrofuran and acetonitrile (Merck) were used as eluents. |
| Affinity data for this assay | |
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