| Assay Method Information | |
| | Fluorescence Polarization Assay (FPA) |
| Description: | Briefly, reaction mixes (100 µL) containing 20 mM HEPES (pH 7.3), 50 mM KCl, 2 mM DTT, 5 mM MgCl2, 20 mM Na2MoO4, 0.01% Triton X-100 with 0.1 mg/mL BSA, 50 nM of recombinant Hsp90α protein, 5 nM of GA-FITC, and varying concentrations of tested compounds or GA were added in a low-binding black 96-well plates. After incubation for 5 h, plates were read on Omega POLARstar Multi-Mode Microplate Reader with excitation at 485 nm and emission at 520 nm. |
| Affinity data for this assay | |
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