| Assay Method Information | |
| | Beta-glucuronidase Inhibition Assay |
| Description: | β-Glucuronidase activity was determined by measuring the absorbance at 405 nm of p-nitrophenol formed from the substrate by the spectrophotometric method. The total reaction volume was 250 µL. DMSO (100%) was used to dissolve the compound (5 µL), which become 2% in the final assay (250 µL) and the same conditions were used for standard (D-saccharic acid 1,4-lactone). The reaction mixture contained 185 µL of 0.1 M acetate buffer, 5 µL of test compound solution, 10 µL of enzyme solution was incubated at 37 °C for 30 min. The plates were read at 405 nm after the addition of 50 µL of 0.4 mM p-nitrophenyl-β-D-glucuronide. All assays were run in triplicate. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |