| Assay Method Information | |
| | Alpha-glucosidase Inhibition Assay |
| Description: | A 100 µL assay mixture containing 70 µL phosphate buffer saline (50 mM at pH 6.8), 10 µL corresponding test compounds dissolved in methanol/DMSO (0.5 mM) and 10 µL (0.057 units) α-glucosidase enzyme were mixed, pre-incubated for 10 min at 37C and the abosrbance was recorded at 400 nm. The reaction was initiated by the addition of 10 µL p-nitrophenyl-α-D-glucopyranoside (substrate, 0.5 mM/well). |
| Affinity data for this assay | |
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