| Assay Method Information | |
| | LanthaScreen TR-FRET Assay |
| Description: | The assay has been run at room temperature on a liquid handling robot. To the assay plates containing 50 mL compound or control solutions, 4.5 uL of solution A (50 mM Tris-HCl pH7.4, 2.0m MDTT, 0.05% Tween20, 0.02 mM Na3VO4) including a generic concentration of 2.0 uM ATP was added per well, followed by 4.5 uL of solution B (0.5% BSA) including a generic concentration of 50 nM poly(EAY) to give 9.05 uL of a reaction volume with final concentrations of 2.0 uM ATP, 50 nM poly(EAY), 25 mM Tris-HCl pH7.4, 1.0 mM DTT, 0.025% Tween20, 0.01 mM Na3VO4, 0.025% BSA as well as specific concentration of the respective enzyme and individual concentrations of divalent cations: (FGFR-3-K650E) 0.2 nM GST-FGR-3-K650E, 3.0 mM MgCl2, (KIT) 36.6 nM GST-KIT, 10 mM MnCl2, (RET) 0.11 nM GST-Xa-RET, 1.0 mM MnCl2, 10 mM MgCl2, (LCK) 3.3 nM His-LCK, 10 mM MnCl2. (KDR) 0.38 nM GST-KDR, 10 mM MgCl2, 1.0 mM MnCl2, (PDGFaV561D) 4.4 nM GST-PDGFRaV561D, 10 mM MnCl2. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |