Assay Method Information | |
| Inhibitory Assay |
Description: | Enzymatic activity of enzyme was evaluated using pNPP assay. Briefly, a reaction mixture (100 µL) containing 50 mM MOPS (pH 6.5), 2 mM pNPP and recombinant enzymes was monitored at 405 nm for 2 min at 30 °C. |
Affinity data for this assay | |
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