| Assay Method Information | |
| | Z-LYTE biochemical assay |
| Description: | To the assay kit, 2.5 µL of different concentrate of the test compounds, Pazopanib or water (control) were added and incubated for 1 h at room temperature followed by the addition of 5 µL development agent. The reaction was stopped by the addition of 5 µL stop reagent. The fluorescence intensity at 445 and 520 nm were monitored and the standard inhibitory reference compound was Staurosporine. |
| Affinity data for this assay | |
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