| Assay Method Information | |
| | HCV Protease Inhibition Assay |
| Description: | The enzymatic assay was performed in assay buffer containing the enzyme complex (protease and NS4A-derived peptide), substrate DDIVPCSMSYTW/biotin-DDIVPCSMSY[25I]TW, and various concentrations of inhibitor. After reaction was terminated, the separation of substrate and products was achieved by adding avidin coated agarose beads to the assay mixture followed by filtration. A non-linear curve fit using the Hill model was applied to the percentage inhibition-concentration data and 50% effective concentration (IC) was calculated through the use of SAS (Statistical Software System, SAS Institute Inc., Gary, N.C.). |
| Affinity data for this assay | |
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