| Assay Method Information | |
| | ChEMBL_2345192 |
| Description: | Inhibition of human N-terminal 6His-tagged BRD4 BD2 (351 to 457 residues) expressed in Escherichia coli BL21-CodonPlus (DE3) cells using biotinylated H4 peptide as substrate preincubated for 30 mins followed by substrate addition and measured after 30 mins by AlphaScreen assay |
| Affinity data for this assay | |
|---|---|
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