| Assay Method Information | |
| | ChEMBL_1907071 (CHEMBL4409429) |
| Description: | Inhibition of recombinant human C-terminal His8-tagged IDH1 R132H mutant expressed in Escherichia coli BL21 assessed as reduction in NADPH consumption using alpha-ketoglutarate as substrate preincubated for 60 mins followed by substrate addition measured for 60 mins in presence of NADPH by diaphorase/resazurin-based assay |
| Affinity data for this assay | |
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