| Assay Method Information | |
| | ChEMBL_1845622 (CHEMBL4346049) |
| Description: | Inhibition of recombinant human GST-tagged p300 HAT domain (1195 to 1673 residues) expressed in Escherichia coli BL21 codon plus cells using Histone H3 peptide (1 to 21) and [3H]acetyl-CoA as substrate preincubated with enzyme for 10 mins followed by substrate addition and measured after 30 mins by scintillation counting method |
| Affinity data for this assay | |
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