| Assay Method Information | |
| | GTP-gamma-S Binding Assay |
| Description: | A GTP-gamma-S CB1 functional assay was used to assess the potency of the pyrazine derivatives. [35S]GTP-gamma-S binding assays were performed at 30 deg C fro 45 min in membrane buffer containing membrane protein, GDP and [35S]GTP-gamma-S in the presence of test compounds. Non specific binding was determined in the presence of 20 uM GTP-gamma-S. The reaction was terminated by addition of ice cold wash buffer followed by rapid filtration under vacuum through Printed Filtermat A glass fibre filters (Wallac) using a Micro 96 Harvester. The bound radioactivity was determined using a 1450 Microbeta Trilux (Wallac) scintillation counter. |
| Affinity data for this assay | |
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