| Assay Method Information | |
| | Cell-Based Assay for Amyloid-beta Accumulation |
| Description: | H4 human neuroglioma cells expressing beta-APP were aliquoted into a 96-well plate, and after attachment the medium was replaced with the conditioned medium containing test compounds. After an overnight incubation, the plates were evaluated for the presence of amyloid-beta (A-beta) in a sandwich ELISA using a monoclonal C-terminal A-beta 40 specific capture antibody and an HRP labeled monoclonal antibody to the N-terminus of A-beta for detection. The plates were read at 450 nm. IC50s are means of two experiments, with 12 drug concentrations in each experiment; intra-assay variance was less than 10% |
| Affinity data for this assay | |
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