| Assay Method Information | |
| | IKK Inhibition Assay |
| Description: | The inhibitory effect of test compounds on kinase activity was measured using a biotinylated I-Kappa-B-alpha peptide and increasing doses of inhibitors (7 point IC50 curves). The incorporation of [gamma-33P] ATP was measured using a Top count NXT (Packard Instrument Co). |
| Affinity data for this assay | |
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