| Assay Method Information | |
| | RET Kinase Inhibition Assay |
| Description: | The biochemical activity of compound was determined by incubation with Ret kinase and the substrate in the presence of ATP/ [gamma-33P] ATP. After in cubation, the reaction was stopped and the phosphorylated substrate was spotted onto phosphocellulose paper, and filter- bound radioactivity was quantified by scintillation counter. |
| Affinity data for this assay | |
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