| Assay Method Information | |
| | In Vitro Enzyme Inhibition |
| Description: | Inhibition of WNV protease activity was measured using recombinant WNV protease, which first incubated with inhibitor in 96-well plates. Catalysis was initiated by adding the substrate. Cleavage of the pNA chromophore from substrate produced a color change monitored at 405 nm. Optical density was measured every 30 s for 10 min by a Spectromax 250 reader, and Ki values were determined by Graphpad Prism. |
| Affinity data for this assay | |
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