| Assay Method Information | |
| | TrkB Enzyme Assay |
| Description: | TrkB kinase activity was measured against its ability to phosphorylate synthetic tyrosine residues within a generic polypeptide substrate using homogeneous time-resolved fluorescence (HTRF) technology. Reaction products are detected with the addition of strepavidin-linked and phosphotyrosine-specific antibodies using the Tecan Ultra Evolution microplate fluorometer after additional 3 h incubation at room temperature. |
| Affinity data for this assay | |
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