| Assay Method Information | |
| | Phosphatase Inhibition Assay |
| Description: | The activity of PTP1B enzyme was assayed with DiFMUP as substrate. Hydrolysis of substrate was monitored on a Victor V plate reader (Wallac). Kinetic measurements were measured using 3-5 concentrations of inhibitor around the IC50 over a range of substrate concentrations (3-500 uM). Data were used to determine Km/Ki/competitive inhibition using GraphPad Prism software. |
| Affinity data for this assay | |
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