| Assay Method Information | |
| | PRMT5 Chemiluminescent Assay |
| Description: | The PRMT5 activity is measured by using “PRMT5 Chemiluminescent Assay Kit” from BPS Bioscience, Catalog Number 52002L as per the instructions of the manufacturer. Briefly, the PRMT5 enzyme is incubated with S-adenosylmethionine in a 96-well plate precoated with histone H4 peptide substrate. Next, a highly specific antibody that recognizes methylated R3 residue of Histone H4 is added followed by a horseradkish peroxidase-labeled (HRP-labeled) secondary antibody. Detection is done by the addition of the HRP substrate to produce chemiluminescence that can be measured quantitatively.Compounds were serially diluted 3-fold and dosed from 10 uM down to 0 uM. Compounds were tested in duplicates to generate an eight-point dose response for the calculation of their IC50s. |
| Affinity data for this assay | |
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