| Assay Method Information | |
| | ADP-Glo™ Kinase Assay |
| Description: | ADP-Glo kinase assay was performed as described by the manufacturer's protocol. Briefly, the kinase assay was performed with a 5 mixture of liver PI/ATP/purified PI3KC2α for 20 min in kinase reaction buffer described above. To deplete the remaining ATP from the reaction, 5 μl of Glo™ reagent was added to the kinase reaction mixture and incubated for 40 min. For detection, the generated ADP is re-converted into ATP and converted into light by the Ultra-Glo™ luciferase in kinase detection reagent. The luminescent signal positively correlates with kinase activity. All reactions are performed in Corning® 384 well-white plates. |
| Affinity data for this assay | |
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