| Assay Method Information | |
| | LanthaScreen LRRK2 Biochemical Activity Assay |
| Description: | The ability of a compound of Formula (I) to inhibit LRRK2 kinase activity is measured using a LanthaScreen™ kinase activity assay. In general, in a LanthaScreen™ kinase activity assay, kinase, fluorescein-labeled substrate, and ATP are allowed to react. Then EDTA (to stop the reaction) and terbium-labeled antibody (to detect phosphorylated product) are added. In a LanthaScreen™ kinase reaction, the antibody associates with the phosphorylated fluorescein labeled substrate resulting in an increased TR-FRET value. The TR-FRET value is a dimensionless number that is calculated as the ratio of the acceptor (fluorescein) signal to the donor (terbium) signal. The amount of antibody that is bound to the tracer is directly proportional to the amount of phosphorylated substrate present, and in this manner, kinase activity can be detected and measured by an increase in the TR-FRET value. |
| Affinity data for this assay | |
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