| Assay Method Information | |
| | Sophion QPatch assay |
| Description: | Electrophysiological characterization of Cav1.2 activators using Cav1.2-HEK293 (AUX) cell line and QPatch 24 hours prior an electrophysiology experiment, doxycycline (1 μg/ml) was added to the growth medium (Table 1), and 25 UM of verapamil was co-applied to prevent calcium influx triggered cell death. Cell confluency should reach 70%-80% right before the experiment. To harvest cells (from a T175 cm2 flask as an example), growth media were removed completely, and the cells were rinsed with 10 ml of D-PBS. D-PBS was aspirated, and 10 ml of Detachin (Genlantis) was added and the plate was placed in a 37° C. incubator for 10 minutes. Detached cells were placed into a 15 ml conical tube and spun at 1000 rpm for 2 minutes. Supernatant was removed, and cells were re-suspended in QPatch complete media (Table 2) to desired cell density of 1.5-3 million cells per QPatch run. Each experimental run uses 1.5 mL of cells. |
| Affinity data for this assay | |
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