| Assay Method Information | |
| | Inhibitory Concentration (IC50) to UDP-2,3-diacylglucosamine hydrolase (LpxH) |
| Description: | The inhibitory potency of molecules to UDP-2,3-diacylglucosamine hydrolase (LpxH) was determined using the UMP/CMP-Glo™ Glycosyltransferase Assay kit. LpxH hydrolyzes the pyrophosphate bond of UDP-2,3-diacylglucosamine (UDP-DAG) to yield 2,3-diacylglucosamine 1-phosphate (lipid X) and UMP. The assay kit could quantify the UMP production by convert the UMP to ATP and generate light in a luciferase reaction. The compound's effect on the LpxH activity is detected by measuring the light using a luminometer (Envision). |
| Affinity data for this assay | |
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