| Assay Method Information | |
| | In vitro Kinase Assay |
| Description: | Lanthascreen Eu kinase binding assays were conducted for Cdk14/CycY largely as performed in the commercial assay service by Life Technologies, but included a 30 minute pre-incubation step of the kinase with candidate compounds to facilitate covalent bond formation. The results of the assays are provided in Table 1 above and Tables 2A, 2B, 2C, and 2D below, in the column “CDK14 IC50.”Lanthascreen Eu kinase binding assays were conducted for CDK16/CycY at Life Technologies. The results of the assays are provided in Table 1 above and Tables 2A, 2B, 2C, and 2D below, in the columns “CDK2 IC50” and CDK16 IC50.” Z'LYTE kinase assays were conducted for CDK2/CycA at Life Technologies using Km ATP concentrations. Cdk14 33P kinase assays were performed by Reaction Biology Corp. The results of the assays are provided in Tables 2A, 2B, 2C, and 2D below, in the column “IC50 CDK14 33P kinase assay.” |
| Affinity data for this assay | |
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