| Assay Method Information | |
| | Evaluation of binding activity for human 5-HT1A receptor, human 5-HT2A receptor, and human D2 receptor |
| Description: | Binding inhibition rate for 5-HT1A receptor (%)=100−100×{(Binding amount of [3H]8-OH-DPAT in the presence of test compound)}−(Binding amount of [3H]8-OH-DPAT in the presence of 10 μmol/L 8-OH-DPAT) }/{(Binding amount of [3H]8-OH-DPAT in the absence of test compound)}−(Binding amount of [3H]8-OH-DPAT in the presence of 10 μmol/L 8-OH-DPAT)}Binding inhibition rate for 5-HT2A receptor (%)=100−100×{(Binding amount of [3H]Ketanserin in the presence of test compound)}−(Binding amount of [3H]Ketanserin in the presence of 10 μmol/L Mianserin)}/{(Binding amount of [3H]Ketanserin in the absence of test compound)}−(Binding amount of [3H]Ketanserin in the presence of 10 μmol/L Mianserin)}Binding inhibition rate for D2 receptor (%)=100−100×{(Binding amount of [3H]Spiperone in the presence of test compound)}−(Binding amount of [3H]Spiperone in the presence of 10 μmol/L Spiperone)}/{(Binding amount of [3H]Spiperone in the absence of test compound)}−(Binding amount of [3H]Spiperone in the presence of 10 μmol/L Spiperone)} |
| Affinity data for this assay | |
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