| Assay Method Information | |
| | Receptor Binding Assay |
| Description: | In general, the results are expressed as a percent of control specific binding:measuredspecificbindingcontrolspecificbinding×100and as a percent inhibition of control specific binding:100-(measuredspecificbindingcontrolspecificbinding×100)obtained in the presence of the test compounds.The IC50 values (concentration causing a half-maximal inhibition of control specific binding) and Hill coefficients (nH) are determined by non-linear regression analysis of the competition curves generated with mean replicate values using Hill equation curve fitting:Y=D+[A-D1+(C/C50)nH]where Y=specific binding, A=left asymptote of the curve, D=right asymptote of the curve, C=compound concentration, C50=IC50, and nH=slope factor. This analysis was performed using in-house software and validated by comparison with data generated by the commercial software SigmaPlot® 4.0 for Windows® (© 1997 by SPSS Inc.). The inhibition constants (Ki) were calculated using the Cheng Prusoff equation:Ki=IC50(1+L/KD)where L=concentration of radioligand in the assay, and KD=affinity of the radioligand for the receptor. A Scatchard plot is used to determine the KD. |
| Affinity data for this assay | |
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