| Assay Method Information | |
| | Inhibitory Effects on Activities of Cytochrome P450 Isozymes |
| Description: | Inhibitory Effects on Activities of Cytochrome P450 Isozymes (CYP1A2, CYP2C9, CYP2C19, CYP2D6, and CYP3A4) from Human Liver Microsomes. A total of six specific probe substrates of six isozymes of CYPs, i.e., α-Naphthoflavone (CYP1A2), Sulphaphenazole (CYP2C9), Ticlopidine (CYP2C19), Quinidine (CYP2D6), Ketoconazole (CYP3A4), and Montelukast (CYP2C8) were co-incubated with recombinant hepatic drug enzymes CYP1A2, CYP2C9, CYP2C19, CYP2D6, CYP3A4, and CYP2C8 and test compounds, respectively. Nicotinamide adenine dinucleotide phosphate (NADP+), D-glucose-6-phosphate (G6P) and glucose-6-phosphate dehydrogenase (G6DHP) were added to start the reaction. At the end of the reaction, the corresponding half maximal inhibitory concentrations (IC50) were calculated by the fluorescence detection method (Ex490 nm/Em520 nm). |
| Affinity data for this assay | |
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