| Assay Method Information | |
| | Inhibitory Effects on Human Renin Activity Assay |
| Description: | Briefly, human recombinant renin (from the kit) was incubated with the compound of the disclosure or buffer for 30 min at 37° C. before substrate addition. Fluorescence intensity was measured at 530 nm after an incubation of 15 min at 37° C. Data was analyzed by Prism and fitted into a sigmodal curve for IC50 calculation. |
| Affinity data for this assay | |
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