| Assay Method Information | |
| | Enzyme Inhibition Assay |
| Description: | For enzyme inhibition assays, compounds were tested in range of ten concentrations from 10 uM to 0.0005 uM using purified enzymes and the Hotspot kinase assay. Reaction conditions were 1 uM ATP, one hour incubation with inhibitor, and kinase activity detected using 33-ATP phosphorylation of an appropriately selected peptide substrate. Dose-response curves were fit using Prism, and the IC50, the concentration at which enzyme inhibition is 50% of maximal inhibition, was determined. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |