| Assay Method Information | |
| | GGDPS Enzyme assay |
| Description: | Recombinant GGDPS was obtained from MyBioSource (San Diego, CA). Recombinant enzyme (20 nM GGDPS) was incubated with assay buffer (50 mM Tris-HCl, pH 7.7, 20 mM MgCl 2, 5 mM TCEP, 5 ⁇ g/mL BSA) and test compounds for 10 minutes at room temperature. The reaction was initiated by the addition of 10 ⁇ M FPP and 10 ⁇ M [ 14 C]-IPP and was carried out at 37° C. for 30 minutes. The reaction was stopped by the addition of saturated NaCl. Radiolabeled GGPP was extracted with n-butanol and counted via liquid scintillation counting. CompuSyn software (ComboSyn, Inc.) was used to analyze the concentration response curves and determine the IC50 values. |
| Affinity data for this assay | |
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