| Assay Method Information | |
| | CDK9 Inhibition |
| Description: | CDK9 activity assays were performed by Nanosyn using a microfluidics mobility shift platform. In this assay, a fluorescently labeled substrate is incubated with the enzyme in the presence of test compounds. Samples are run through microfluidic chips, separating them based on differences in charge; this separates the more positive substrate from the more negative product. Enzyme activity is measured through comparison of the fluorescence from substrate and product peaks. |
| Affinity data for this assay | |
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