| Assay Method Information | |
| | Human VAP-1 Enzyme Activity Assay |
| Description: | Amplex Red Monoamine Oxidase Kit (Invitrogen #A12214) was used to determine the inhibitory effects of the sample on VAP-1 enzyme activity. 100 nL Test compound (the solvent was DMSO) that had been gradiently diluted was added to a 384-well plate. 25 uL 10 nM VAP-1 enzyme solution was added, and the solution was incubated at room temperature for 30 minutes. The substrate mixture which had been added with VAP-1 enzyme (200 uM Amplex Red, 1 U/mL HRP, 1 mM Benzylamine) was incubated at room temperature for 60 minutes. After the incubation, the fluorescence signal was read with a microplate reader Envision (excitation light wavelength 530-560 nm, emission light wavelength 590 nm). The fluorescence signal value after removing the background signal was analyzed with Prism software. |
| Affinity data for this assay | |
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