| Assay Method Information | |
| | Z'-LYTE Biochemical Assay |
| Description: | The experiment were carried out according to the instructions of the Z′-LYTE kinase test kit-tyrosine 1 peptide. Reagent preparation: 1.33 kinase buffer: 5 kinase buffer was diluted with water to 1.33 kinase buffer; an enzyme solution: the kinase was dissolved in 1.33 kinase buffer with the final working concentration being 3.32 nM; a short peptide solution: a short peptide stock solution (1 mM dissolved in DMSO) was dissolved in 1.33 kinase buffer with the final working concentration being 2 uM; Z′-LYTE Tyr01 phosphorylated short peptide solution, 0.6 ul of stock solution (1 mM dissolved in DMSO) was dissolved in 149.4 ul of 1.33 kinase buffer; an ATP solution: an ATP stock solution (10 mM aqueous solution) was dissolved in 1.33 kinase buffer with the final working concentration being 32 uM; a color-developing solution: color-developing solution B was dissolved in color-developing buffer with the final working concentration being 1 color-developing solution; 4 compound preparation: the compound was diluted in 3-fold gradient concentration to finally obtain 4% DMSO aqueous solution containing different concentrations of the compound, with the final working concentration being 3000, 1000, 333.33, 111.11, 37.04, 12.35, 4.12, 1.37 nM, 8 concentration points in total. |
| Affinity data for this assay | |
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