| Assay Method Information | |
| | Ki Estimation |
| Description: | Ligand binding was measured by fluorescence polarization of a fluorescein isothiocyanate (FITC) labeled probe (as described by Bollini, et al 2002) in conjunction with recombinant full-length FKBP12 and FKBP52. Binding saturation experiments were performed to determine the probe Kd for FKBP12 or FKBP52 using GraphPad software. Ligand displacement from FKBP12 or FKBP52 was measured by fluorescence polarization in the presence of various compound concentrations. IC50 for each compound was determined using GraphPad software. The IC50 value for each compound was used to estimate the Ki using the equations described by Nikolovska-Coleska, et al 2004. |
| Affinity data for this assay | |
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