| Assay Method Information | |
| | standard FabI enzyme assay |
| Description: | To probe the effects of varying ring systems and amine placement on FabI target engagement, the apparent inhibition constants (Kiapp) for select compounds were determined using purified FabI. The goal of these studies was two-fold, to comparatively assess fabimycin, Debio-1452-NH3, and Debio-1452, and to also assess differential activity against the E. coli and A. baumannii versions of FabI. FabI from E. coli and A. baumannii were recombinantly expressed and purified, and the ability for each compound to inhibit the activity of these enzymes was evaluated using a standard FabI enzyme assay. |
| Affinity data for this assay | |
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