| Assay Method Information | |
| | Qube Assay |
| Description: | NaV1.5 current measurements on Qube were obtained as follows: NaV1.5 currents were elicited with a 20 second 3 Hertz pulse train in the control condition (DMSO only) and after compound addition. The pulse train consisted of sixty 20 millisecond test pulses to 0 millivolt from a holding potential of +-80 millivolt (mV). The average peak currents elicited by the last 3 test pulses were used to determine IC50 values for NaV1.5 inhibition. The following buffers were used for the Qube recordings: External buffer for NaV1.8 Qube recording: 150 NaCl, 2 CaCl2, 5 KCl, 1 MgCl2, 10 HEPES, 12 Dextrose; External buffer for Qube NaV1.5 recording: 120 N-Methyl-D-Glucamine, 40 NaCl, 1 KCl, 2.7 CaCl2), 5 HEPES, 0.5 MgCl2; and Internal buffer for Qube recording: 120 CsF, 30 CsCl, 10 EGTA, 5 HEPES, 5 NaF, 2 MgCl2. |
| Affinity data for this assay | |
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