| Assay Method Information | |
| | LSD1 Inhibition of the Crystal Form A of the Compound III |
| Description: | 1.1 Experimental Purpose:The experimental purpose was to evaluate IC50 of the crystal form A of the compound III at 10 concentrations against LSD1. The experiment was conducted in duplicate with an initial concentration of 10 μM diluted in a gradient of 3 times, and it was repeated twice at different dates.1.2 Test Conditions:LSD1 buffer composition: 50 mM Tris-HCl, pH 7.5, 0.05% CHAPS, 1% DMSO.Reaction time: reacted at room temperature for 1 hourReaction Process:1.2.1 Adding enzyme to a freshly prepared buffer1.2.2 Adding a DMSO solution of the compound to the enzyme mixture using Acoustic Technology (Echo 550, LabCyte Inc. Sunnyvale, CA) at nL level, and incubating at room temperature for 30 minutes1.2.3 Adding the substrate to a freshly prepared buffer1.2.4 Incubating at room temperature for 1 hour1.2.5 Preparing to test the mixture1.2.6 Using Perkin Elmer Envision to read data1.2.7 Using Excel and GraphPad Prism software to analyze data |
| Affinity data for this assay | |
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