| Assay Method Information | |
| | In-Vitro Assay |
| Description: | The inhibitory effect of the compounds on the enzyme activity of recombinant protein Wee-1 was determined by HTRF. The procedures are as follows: After DMSO or serially diluted compounds (up to 200 nM, 1:5 diluted) and recombinant proteins were co-incubated in a kinase buffer at 37° C. for 30 min before Fluorescein-PolyGAT and ATP were added, and the reaction was initiated by addition of a substrate. After incubation at room temperature for 90 min, an antibody and a detection solution were added, and after further incubation at room temperature for 60 min, fluorescence values were detected (excitation wavelength: 340 nm, emission wavelengths: 495 and 520 nm). The 520 nm/495 nm fluorescence intensity ratio value was calculated, and compared with that of the DMSO group, and then the inhibition percentages and IC50 values of the compounds were calculated. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |