| Assay Method Information | |
| | IRAK4 Kinase Inhibitory Activity Assay |
| Description: | The inhibitory activity (IC50) of the compound on IRAK4 kinase under Km ATP was detected by mobility shift assay (MSA). Ten drug concentration gradients were set (initial concentration 1 μM, 3-fold dilution, 2 duplicate wells per concentration). IRAK4 kinase was added to the kinase buffer solution, which was transfered to a test plate, and then FAM-labeled peptide and ATP (37 μM) were added. After incubation at 28° C. for a period of time, 10 μL of termination buffer was added to terminate the reaction. The conversion rate data was read with Caliper, and then the conversion rate was converted into inhibition rate data. According to the inhibition rate data of each concentration, the IC50 of half inhibitory concentration was calculated by Logit method (Table 3). |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |