| Assay Method Information | |
| | Enzymatic Assay for Mpro |
| Description: | The SARS-CoV-2 3CLPro (Mpro), MBP-tagged Assay Kit (BPS Biosciences, San Diego, CA, USA) was used to measure 3CL Protease (Mpro) activity for screening and profiling applications. The 3CL inhibitor GC376 was included as a positive control and also used as a standard. The assay was performed according to the protocol provided by the manufacturer with the concentration of compounds tested being 10−7 M. |
| Affinity data for this assay | |
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