| Assay Method Information | |
| | Enzyme Activity Test |
| Description: | Serum was diluted with diluent (1:23). Drugs were added to the diluted serum with 8 concentration gradients of up to 10 mM or 50 mM in a 5-fold serial dilution. The mixture was incubated at room temperature for 15 min. The mixture of compound and serum was added to a 96-well plate (100 μL/well) provided in the kit, and activated at 37° C. for 1 hour. The plate was washed three times with wash buffer. The plate was added with detection antibody (100 μL) provided in the kit and incubated at room temperature for 30 min. The plate was washed three times with wash buffer. The plate was added with substrate (100 μL) and incubated at room temperature for 30 min. The absorbance was detected at 405 nM on a microplate reader. |
| Affinity data for this assay | |
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